What are the Limitations of Tissue Clarity Techniques?
Despite their advantages, enhanced tissue clarity techniques also have some limitations. These include:
- Compatibility with Antibodies: Some clearing agents may interfere with antibody binding, making immunostaining challenging. - Tissue Shrinkage: Certain clearing protocols can cause tissue shrinkage or distortion, affecting the accuracy of spatial measurements. - Fluorescence Quenching: Prolonged exposure to clearing agents or imaging conditions may lead to the quenching of fluorescent signals, limiting the duration of imaging experiments.
