What are the key techniques used in Histology for studying development?

Several techniques are central to histological studies of development:

Histochemical staining: Different stains are used to highlight specific cellular components. For example, Hematoxylin and Eosin (H&E) staining is commonly used to distinguish between cellular nuclei and cytoplasm.
Immunohistochemistry: This technique uses antibodies to detect specific proteins within tissues, providing insights into the presence and distribution of various molecules critical for development.
In situ hybridization: This method allows for the visualization of specific nucleic acid sequences within tissues, helping to identify gene expression patterns during development.
Electron microscopy: Provides ultra-high resolution images of tissue structures at the cellular and subcellular levels, offering detailed insights into developmental processes.