1.
Specimen Handling and Identification: Proper
handling and accurate identification of tissue samples are critical. Each specimen should be correctly labeled and tracked through the entire process.
2.
Fixation: The fixation process preserves tissue morphology and prevents degradation. The choice of fixative, typically
formalin, and fixation time are crucial factors.
3.
Processing: Ensuring that the tissue is adequately dehydrated, cleared, and embedded is vital for producing high-quality sections.
4.
Sectioning: Consistent and thin sections, generally 3-5 micrometers, are essential for clear microscopic examination.
5.
Staining: Staining enhances tissue contrast. Standardization of staining protocols, including Hematoxylin and Eosin (H&E) staining, is necessary.
6.
Microscopic Examination: Regular calibration of microscopes and ensuring they are in good working condition is part of QC.
