Hands-on training in histology typically includes several key components:
1. Sample Collection and Fixation Participants learn how to collect and fix tissue samples to preserve cellular structure and prevent degradation. Fixation techniques such as using formalin or other fixatives are covered.
2. Tissue Processing This involves dehydrating, clearing, and embedding tissues in paraffin wax. Trainees use automated tissue processors to streamline this process.
3. Sectioning Sectioning is the process of cutting thin tissue slices using a microtome or cryostat. Hands-on training ensures that participants can produce consistent, high-quality sections for further analysis.
4. Staining Participants learn various staining techniques such as Hematoxylin and Eosin (H&E), special stains, and immunohistochemistry. Staining is essential for highlighting different cellular components.
5. Slide Analysis Analyzing stained slides under a microscope is a critical skill. Trainees gain experience in identifying normal and pathological structures, enhancing their diagnostic capabilities.
