An LSCM system comprises several essential components: 1. Laser Source: Provides the excitation light for fluorescence. 2. Scanning Mirrors: Direct the laser beam across the specimen. 3. Pinhole Aperture: Blocks out-of-focus light to enhance image clarity. 4. Objective Lens: Focuses the laser light onto the specimen. 5. Detectors: Capture the emitted fluorescence. 6. Computer: Controls the system and processes the acquired images.
