What are the Disadvantages of Using Ethidium Bromide?
Toxicity: Ethidium bromide is a potent mutagen and is considered hazardous. Proper safety measures, such as wearing gloves and protective eyewear, are essential. Disposal: Disposal of EtBr requires special procedures to avoid environmental contamination, complicating waste management. Potential for Background Staining: Non-specific binding may lead to background staining, which can complicate the interpretation of results.
