What are the Different Types of Advanced Microscopy?
Several types of advanced microscopy are commonly used in histology:
Confocal Microscopy: This technique uses point illumination and a spatial pinhole to eliminate out-of-focus light, providing high-resolution, three-dimensional images of tissues. Fluorescence Microscopy: By labeling tissues with fluorescent dyes or proteins, this method allows for the visualization of specific cellular components with high specificity and sensitivity. Electron Microscopy: Techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) provide extremely high resolution, revealing ultrastructural details at the nanometer scale. Multiphoton Microscopy: This technique uses multiple photons to excite fluorescent molecules, allowing imaging of living tissues at greater depths than confocal microscopy.
