heat induced epitope retrieval (hier)

What are the Common Solutions Used in HIER?

Several retrieval solutions are commonly used in HIER, including:
Citrate buffer (pH 6.0)
Tris-EDTA buffer (pH 9.0)
EDTA buffer (pH 8.0)
The choice of buffer depends on the specific antigen and the antibody used. For example, citrate buffer is effective for a wide range of antigens, while Tris-EDTA buffer is often used for more robust antigens.

Frequently asked queries:

What is Heat Induced Epitope Retrieval (HIER)?
Why is HIER Necessary?
How Does HIER Work?
What are the Common Solutions Used in HIER?
What are the Steps Involved in HIER?
What are the Factors Affecting HIER?
What are the Advantages of HIER?
Are There Any Limitations to HIER?
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What are Software Bugs in Histology?
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