heat induced epitope retrieval (hier)

What are the Common Solutions Used in HIER?

Several retrieval solutions are commonly used in HIER, including:
Citrate buffer (pH 6.0)
Tris-EDTA buffer (pH 9.0)
EDTA buffer (pH 8.0)
The choice of buffer depends on the specific antigen and the antibody used. For example, citrate buffer is effective for a wide range of antigens, while Tris-EDTA buffer is often used for more robust antigens.

Frequently asked queries:

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Why is HIER Necessary?
How Does HIER Work?
What are the Common Solutions Used in HIER?
What are the Steps Involved in HIER?
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What are the Advantages of HIER?
Are There Any Limitations to HIER?
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