What are the Common Methods for Enhancing Contrast?
Several methods are employed to enhance contrast in histological samples:
Staining: The most common method where dyes or stains are used to add color to tissues, making different structures more distinguishable. Phase-Contrast Microscopy: Enhances contrast by exploiting differences in the refractive index of tissues. Differential Interference Contrast (DIC) Microscopy: Uses polarized light to produce high-contrast images of unstained samples. Fluorescence Microscopy: Uses fluorescent dyes or proteins to label specific structures within the cells.