What Are the Common Artifacts in Histology and How to Prevent Them?
Artifacts are distortions or alterations in tissue appearance that can complicate diagnosis. Common artifacts include:
Fixation artifacts: Caused by improper fixation, leading to shrinkage or swelling. Sectioning artifacts: Tears or folds in tissue sections, often due to blunt microtome blades. Staining artifacts: Uneven staining or precipitate formation, often due to poor technique or reagent quality.
Preventing artifacts involves following standardized protocols, using high-quality reagents, and maintaining equipment properly.
