Despite their importance, metabolic studies face several challenges:
1. Complexity of Metabolic Pathways: Metabolic pathways are highly complex and interconnected, making it difficult to isolate specific processes. 2. Tissue Heterogeneity: Different cell types within a tissue can have varying metabolic activities, complicating the analysis. 3. Technical Limitations: Techniques like autoradiography and in situ hybridization require specialized equipment and expertise.
