What are the Challenges in Achieving Optimal Thickness?
Achieving optimal tissue thickness can be challenging due to several factors:
Tissue Type: Different tissues have varying densities and compositions, making some tissues harder to section uniformly. Fixation: Proper fixation is essential to maintain tissue integrity. Poor fixation can lead to brittleness or excessive softness, complicating sectioning. Dehydration and Embedding: The processes of dehydration and embedding in paraffin wax must be carefully controlled to avoid artifacts that affect sectioning.
