Despite their advantages, 3D cultures also present certain challenges:
Complexity: The complexity of creating and maintaining 3D cultures can be higher compared to traditional 2D cultures. Standardization: There is a need for standardized protocols and materials to ensure reproducibility and consistency across different studies. Cost: The materials and equipment required for 3D cultures can be more expensive, limiting their widespread adoption. Analysis: Analyzing 3D cultures can be more challenging, requiring advanced imaging and computational techniques.
