specificity of stains

What are the challenges associated with stain specificity?

Achieving and maintaining stain specificity can be challenging due to several factors:
Tissue Preparation: Improper fixation or processing can alter the chemical properties of tissues, affecting stain specificity.
Staining Protocols: Variations in staining protocols, such as differences in pH or staining times, can lead to inconsistent results.
Interpretation: The subjective nature of stain interpretation can lead to variability in identifying specific structures.

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