Cytotoxicity studies face several challenges, including:
- [In Vitro vs. In Vivo](https://): Results from cell cultures (in vitro) may not always correlate with effects in whole organisms (in vivo). - [Complexity of Tissues](https://): Tissues consist of various cell types with different susceptibilities, making it difficult to generalize findings. - [Technical Variability](https://): Variations in assay conditions and techniques can impact results. - [Sensitivity and Specificity](https://): Assays must be sensitive enough to detect subtle changes and specific enough to distinguish between different types of cell death.
