Identifying specific cell types can be challenging due to:
Cell Similarity Some cell types have similar morphological features, making them difficult to distinguish. For example, fibroblasts and myofibroblasts can appear very similar under a microscope.
Changes Due to Disease Diseases can alter the morphology and marker expression of cells, complicating identification. For example, cancer cells often lose their typical structure and may express different markers.
Technical Limitations The resolution of microscopes and the specificity of stains or antibodies can limit the ability to accurately identify cells. Advanced techniques like confocal microscopy and mass spectrometry are sometimes required for precise identification.
