Several techniques are used to study germ layers and their derivatives:
Microscopy: Various types of microscopy, including light and electron microscopy, are used to observe the cellular structure of tissues derived from germ layers. Immunohistochemistry: This technique employs antibodies to detect specific proteins, helping to identify cells and tissues from particular germ layers. In Situ Hybridization: This method is used to detect specific nucleic acid sequences, aiding in the study of gene expression in germ layers.