What Are Common Techniques for Ensuring Specificity?
Several histological techniques are designed to ensure high specificity:
- Immunohistochemistry (IHC): This technique uses antibodies to detect specific antigens in tissue sections. High specificity is achieved by using well-characterized antibodies and optimizing staining conditions. - In Situ Hybridization (ISH): ISH is used to detect specific nucleic acid sequences within tissue sections. The use of highly specific probes ensures that the signal corresponds to the target sequence. - Fluorescence In Situ Hybridization (FISH): Similar to ISH but uses fluorescent probes, allowing for the detection and localization of specific DNA or RNA sequences with high specificity.
