1. Improper Fixation: Delaying fixation or using an inappropriate fixative can lead to tissue degradation. 2. Inadequate Processing: Skipping or shortening processing steps can result in poor tissue preservation and difficult sectioning. 3. Poor Sectioning Technique: Using a dull microtome blade or cutting too quickly can cause tissue tearing or uneven sections. 4. Contamination: Touching the tissue with non-sterile instruments or hands can introduce contaminants that affect staining and analysis. 5. Incorrect Staining: Over-staining or under-staining can obscure details, making it difficult to interpret the tissue morphology.
