What Are Common Issues with Incorrect Antibody Concentrations?
Using incorrect antibody concentrations can lead to several issues:
Non-specific binding: High concentrations can cause antibodies to bind non-specifically to unrelated proteins. Weak signals: Low concentrations may not sufficiently bind to the target antigen, resulting in weak or undetectable signals. High background: Excess antibody can increase background noise, making it difficult to distinguish the specific signal.
