Using DNase in histological procedures involves several steps:
1. Preparation: DNase is typically prepared in a buffer containing the necessary cofactors (e.g., Mg²⁺ and Ca²⁺ for DNase I). 2. Application: The enzyme is applied to tissue sections or cell preparations either by incubation at specific temperatures or by direct addition to the sample. 3. Incubation: Incubation times and conditions vary depending on the protocol and the type of DNase used. 4. Termination: The reaction is usually terminated by adding a chelating agent like EDTA to bind divalent cations or by heating the sample.
