The procedure for using citrus-based clearing agents is similar to that of traditional solvents. Here are the general steps: 1. Dehydration: Tissue samples are first dehydrated using a series of ethanol solutions. 2. Clearing: The dehydrated tissues are then immersed in the citrus-based clearing agent. The duration of this step can vary depending on the size and type of tissue. 3. Infiltration: After clearing, the tissues are ready for embedding in paraffin or another embedding medium.
