Choosing the appropriate detection system is vital for achieving accurate results. Factors to consider include the type of microscope available, the desired sensitivity, and the specific application. Chromogenic detection systems, like DAB (3,3'-Diaminobenzidine), are popular for their permanent results and compatibility with brightfield microscopy. Fluorescent detection, on the other hand, offers multiplexing capabilities and is favored for its ability to detect multiple antigens simultaneously.
