How to Prepare Samples for Scintillation Counting?
Sample preparation is a critical step in scintillation counting. Here are the general steps:
1. Labeling: The biological sample is labeled with a radioactive isotope, such as tritium or carbon-14. 2. Homogenization: The labeled sample is homogenized to ensure even distribution of the radioactive isotope. 3. Mixing with Scintillation Fluid: The homogenized sample is mixed with a scintillation cocktail that enhances the detection of light emissions. 4. Measurement: The prepared sample is placed in the scintillation counter for measurement.