Preparation of Nile Blue staining solution involves dissolving a small amount of the dye in distilled water or ethanol. The concentration typically ranges between 0.1% to 1%, depending on the specific application. The staining procedure usually involves the following steps:
Fixation: Fix the tissue sample using an appropriate fixative like formalin to preserve cellular structures. Sectioning: Cut the fixed tissue into thin sections using a microtome. Staining: Immerse the tissue sections in the Nile Blue staining solution for a specified period, usually 10-30 minutes. Rinsing: Rinse the stained sections with distilled water to remove excess dye. Mounting: Mount the sections on slides using a suitable mounting medium for microscopic examination.
