Optimizing IHC protocols is essential to achieve reliable and reproducible results. Key considerations include:
Choosing the right primary antibody with validated specificity for the target antigen. Optimizing antigen retrieval conditions, including pH, temperature, and retrieval time. Using appropriate blocking agents to minimize non-specific binding. Adjusting antibody dilutions and incubation times. Implementing proper controls, including positive and negative controls, to validate the results.
