Implementing blank controls in histological experiments involves several key steps:
Preparation: Prepare your tissue sections as usual, but set aside additional sections for use as blank controls. Omission of Primary Antibody: For negative controls, skip the step where the primary antibody is applied. For reagent controls, exclude the target-specific reagents. Application of Secondary Antibody: Apply the secondary antibody or detection system to the blank control sections to check for non-specific binding. Comparison: Compare the stained sections with the blank controls to identify and quantify any non-specific staining or background noise.
