Several steps should be taken to ensure quality in histological procedures:
Sample Collection: Ensure that the collection of samples is done using sterile and appropriate methods to avoid contamination and degradation. Fixation: Proper fixation of tissues using suitable fixatives like formalin helps in preserving the tissue morphology and prevents autolysis. Embedding: Embedding tissues in paraffin wax or other media should be done carefully to maintain the tissue structure for sectioning. Sectioning: Using a microtome, thin sections should be cut with precision, ensuring uniform thickness for better microscopic examination. Staining: Staining techniques like Hematoxylin and Eosin (H&E) should be standardized to highlight different cell components and structures effectively.
