Selecting an appropriate permeabilization agent depends on several factors:
- Tissue Type: Different tissues have varying lipid compositions and structural complexities. - Target Molecule: The nature of the molecule being detected (protein, nucleic acid, etc.) can influence the choice of permeabilization agent. - Detection Method: The method of detection, whether it be immunofluorescence, flow cytometry, or immunohistochemistry, can also guide the choice of permeabilization agent.
