Choosing the correct isotype control involves matching several characteristics of the primary antibody:
Isotype: The control antibody should have the same isotype (e.g., IgG1, IgG2a) as the primary antibody. Species: The control should be derived from the same species as the primary antibody (e.g., mouse, rabbit). Conjugation: The control antibody should be conjugated to the same fluorochrome or enzyme as the primary antibody.
By matching these properties, you ensure that the isotype control will accurately reflect non-specific binding characteristics.
