high viability

How is Viability Assessed?

Assessing cell viability typically involves a combination of staining techniques and biochemical assays. Common methods include:
- Trypan Blue Exclusion: This dye is excluded by viable cells but penetrates dead cells, allowing for easy differentiation under a microscope.
- MTT Assay: Measures the metabolic activity of cells; viable cells reduce MTT to a purple formazan product.
- Fluorescent Dyes: Such as propidium iodide (PI) and calcein AM, which can differentiate between live and dead cells based on membrane integrity and esterase activity.

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