In histology, titration is performed by gradually adding a reagent of known concentration to the staining solution until the desired endpoint is achieved. This process can be monitored using various indicators, such as pH changes or color shifts, to ensure that the solution reaches the optimal concentration for staining. The steps typically involve:
Preparing a standard solution of known concentration. Adding the titrant to the staining solution while constantly mixing. Observing the endpoint using visual or instrumental indicators.
