How is Tissue Prepared for Histopathological Examination?

The preparation of tissue for histopathological examination involves several steps. Initially, the tissue is obtained through a biopsy or surgical excision. The tissue is then fixed, usually in formalin, to preserve its structure. Following fixation, the specimen is embedded in paraffin wax to provide support for thin sectioning. The tissue sections are then cut using a microtome, placed on glass slides, and stained with various dyes, such as Hematoxylin and Eosin (H&E), to highlight different cellular components.