How is the Cytochrome P450 Epoxygenase Pathway Studied in Histology?
Histological techniques are employed to study the spatial distribution and activity of CYP450 epoxygenases within tissues. These techniques include: - Immunohistochemistry: This method uses antibodies to detect specific CYP450 enzymes in tissue sections. - In situ hybridization: This technique helps visualize the mRNA expression of CYP450 enzymes within tissues. - Western blotting: Although not a histological method per se, it is often combined with histological studies to quantify protein levels in tissue extracts.
