The process involves several advanced techniques and technologies:
Microdissection: Laser capture microdissection is often used to isolate single cells from tissue sections. Immunohistochemistry: This technique uses antibodies to detect specific proteins within individual cells. Fluorescence In Situ Hybridization (FISH): FISH is used to detect and localize the presence or absence of specific DNA sequences on chromosomes. Single Cell RNA Sequencing (scRNA-seq): This method allows for the examination of gene expression at the single-cell level.