The process of serial sectioning involves several steps:
Fixation: The tissue is preserved using a fixative solution to prevent decomposition. Embedding: The fixed tissue is embedded in a solid medium, commonly paraffin wax, to provide support during slicing. Sectioning: A microtome is used to cut the tissue into thin sections, typically ranging from 3 to 10 micrometers in thickness. Mounting: Each section is mounted onto a glass slide for staining and examination under a microscope.
