Ensuring high-quality samples involves several steps:
Proper Fixation: Fixation preserves tissue morphology and prevents degradation. Formalin is a commonly used fixative. Accurate Sectioning: Thin sections (usually 4-5 micrometers) are cut to ensure that cellular details are clearly visible under the microscope. Staining Techniques: Routine stains like Hematoxylin and Eosin (H&E) provide basic tissue architecture, while special stains (e.g., PAS, Masson's trichrome) highlight specific tissue components.
