The process involves several steps: 1. Sample Preparation: The tissue sample is first prepared and cut into small pieces to facilitate rapid cooling. 2. Freezing Medium: The sample is often placed in a freezing medium like OCT compound (Optimal Cutting Temperature compound) which helps protect the tissue. 3. Freezing Device: The sample is then placed in a freezing device, such as a cryostat or a liquid nitrogen-cooled chamber, to rapidly bring the temperature down to around -196°C.
