The process of post fixation involves several steps: 1. Initial Fixation: The tissue is first fixed using a primary fixative like formaldehyde. 2. Rinsing: The fixed tissue is rinsed to remove excess fixative. 3. Post Fixation Treatment: The tissue is then treated with post-fixative reagents, which may vary based on the intended analysis. 4. Dehydration: This step involves passing the tissue through a series of graded alcohols to remove water. 5. Clearing and Embedding: The tissue is cleared using a solvent like xylene and then embedded in a suitable medium like paraffin for sectioning.
