The use of EDTA in tissue processing involves several steps:
Fixation of the tissue sample to preserve cellular structures Immersion of the fixed tissue in an EDTA solution, typically at a pH of 7.0-7.4 Monitoring the decalcification progress by checking the sample's pliability or using chemical tests to detect remaining calcium Rinsing the tissue thoroughly to remove any remaining EDTA, followed by standard tissue processing protocols
