In histology, DSBR can be studied through various techniques, including:
Immunohistochemistry (IHC) IHC can be used to detect the presence and localization of DSBR proteins such as BRCA1 and Rad51 in tissue sections. This technique involves using specific antibodies to bind to these proteins, followed by visualization using chromogenic or fluorescent labels.
Fluorescence In Situ Hybridization (FISH) FISH allows for the visualization of [DNA sequences]() and the detection of chromosomal abnormalities that might result from improper DSBR. It employs fluorescent probes that bind to specific DNA regions, enabling the identification of structural changes.
Comet Assay The comet assay, also known as single-cell gel electrophoresis, is used to measure DNA damage and repair in individual cells. Cells are embedded in agarose gel, lysed, and subjected to electrophoresis. The extent of DNA migration, which resembles a comet, indicates the level of DNA damage.
