The general procedure for DAB staining in IHC involves the following steps:
Prepare the tissue sections and perform antigen retrieval if necessary. Block endogenous peroxidase activity to prevent non-specific staining. Apply the primary antibody specific to the target antigen. Add a secondary antibody conjugated to HRP. Incubate the tissue with the DAB substrate solution. Monitor the development of the brown color under a microscope. Stop the reaction by rinsing with water and counterstain if desired. Dehydrate, clear, and mount the tissue sections for microscopic examination.
