The activity of CDK1 is tightly regulated by several mechanisms, including phosphorylation and dephosphorylation. CDK1 is kept inactive by phosphorylation through Wee1 kinase. Activation occurs when the inhibitory phosphate group is removed by Cdc25 phosphatase, allowing CDK1 to bind cyclin B and become active. Additionally, CDK inhibitors (CKIs) can bind to CDK1 and prevent its activation, adding another layer of regulation.
