There are multiple techniques to perform 3D cell culture. Some of the most common methods include:
Scaffold-based techniques: Cells are grown on a three-dimensional scaffold made of materials like collagen, gelatin, or synthetic polymers. Spheroid cultures: Cells are allowed to aggregate and form spheroids, which can mimic the architecture of tissues like tumors. Hydrogels: These are water-swollen, cross-linked polymer networks that can encapsulate cells, providing a hydrated environment similar to natural tissues. Organoids: These are miniaturized, simplified versions of organs produced in vitro, which can self-organize and mimic the function of real organs.
