pass method

How Does the PAS Method Work?

The PAS method consists of several steps:
1. Oxidation: Tissue sections are treated with periodic acid, which oxidizes the vicinal diols in carbohydrates to form aldehyde groups.
2. Schiff Reagent Application: The tissue is then exposed to Schiff reagent, which reacts with the aldehyde groups to form a magenta compound.
3. Counterstaining: Often, a hematoxylin counterstain is applied to provide contrast and to better visualize cell nuclei.

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