In histology, RNA-seq can be applied to understand the gene expression patterns within different tissue types. The process generally involves the following steps:
Sample collection: Tissue samples are collected and preserved using methods like formalin-fixed paraffin-embedded (FFPE) or fresh-frozen techniques. RNA extraction: RNA is isolated from the tissue samples using various extraction protocols. Library preparation: The extracted RNA is converted into complementary DNA (cDNA) and prepared for sequencing. Sequencing: The cDNA libraries are sequenced using high-throughput sequencing technologies, such as Illumina. Data analysis: The sequencing data is analyzed to identify gene expression levels, alternative splicing events, and novel transcripts.
