Prussian blue stain works by reacting ferric iron (Fe3+) with potassium ferrocyanide. The chemical reaction results in the formation of ferric ferrocyanide, also known as Prussian blue, which is visible under a microscope. The reaction formula can be summarized as: \[ \text{4Fe}^{3+} + 3\text{K}_4\text{[Fe(CN)_6]} \rightarrow \text{Fe}_4[\text{Fe(CN)_6}]_3 \] This reaction specifically highlights areas with ferric iron deposits, providing a stark contrast against the surrounding tissue.
