The staining process involves three primary dyes: Weigert's iron hematoxylin, which stains cell nuclei black; Biebrich scarlet-acid fuchsin, which stains muscle fibers red; and aniline blue or light green, which stains collagen blue or green, respectively. The differentiation of these components is achieved through a series of steps that include fixation, mordanting, staining, and differentiation.
