E3 ubiquitin ligase facilitates the transfer of ubiquitin from an E2 enzyme to the substrate protein, marking it for degradation. The process involves a cascade where ubiquitin is first activated by E1, transferred to E2, and finally attached to the substrate by E3. This ubiquitination can lead to the protein being recognized and degraded by the proteasome or modulate its activity, location, or interactions.
