The process of ChIP-Seq involves several key steps:
Crosslinking: Cells are treated with formaldehyde to crosslink proteins to DNA, preserving the protein-DNA interactions. Chromatin Shearing: The crosslinked chromatin is then sheared into smaller fragments using sonication or enzymatic digestion. Immunoprecipitation: Antibodies specific to the protein of interest are used to selectively precipitate the protein-DNA complexes. DNA Purification: The protein-DNA complexes are reversed, and the DNA is purified for sequencing. Sequencing: The purified DNA is subjected to high-throughput sequencing to identify the genomic locations of the protein-DNA interactions.
